Prokaryotic expression of HLA-B * 2705 heavy chain and identification for its activity / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1028-1031, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-318796
ABSTRACT
In order to investigate the expression of heavy chain of HLA-B * 2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B * 2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a (+)-B * 2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B * 2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B * 2705 fusion protein are obtained as basis for the further studies.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antígeno HLA-B27
/
Classificação
/
Cadeias Pesadas de Imunoglobulinas
/
Alergia e Imunologia
/
Escherichia coli
/
Vetores Genéticos
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Artigo
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