Influence of intra-bone marrow infusion of peripheral hematopoietic stem cells on graft-versus-host disease / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1009-1012, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-318800
ABSTRACT
This study was aimed to explore whether the graft-versus-host disease (GVHD) could be alleviated by intra-bone marrow (IBM) infusion of allogeneic hematopoietic stem cells. Female C57BL/6 mice as recipients received total body irradiation (TBI) 4 Gy on day 0, followed by injection of peripheral hematopoietic stem cells (1 x 10(7)) from mobilized male BALB/c with granulocyte-colony stimulating factor (rhG-CSF), and cyclophosphamide (200 mg/kg) was injected intraperitoneally two days later. The results showed that the incidence and severity of GVHD were more low and alleviative in group IBM-PBSCT than that in group TV-PBSCT (p < 0.05). Y chromosome of donor mice could be detected in the bone marrow of recipient mice. It is concluded that the method of intra-bone marrow infusion is superior to injection via the tail vein in the engraftment of hematopoietic stem cells in terms of stem cell homing while the frequency and severity of GVHD in allogeneic mice decrease.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Fator Estimulador de Colônias de Granulócitos
/
Irradiação Corporal Total
/
Ciclofosfamida
/
Modelos Animais
/
Transplante de Células-Tronco de Sangue Periférico
/
Usos Terapêuticos
/
Doença Enxerto-Hospedeiro
/
Métodos
/
Camundongos Endogâmicos BALB C
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Artigo
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