In vitro differentiation of human bone marrow-derived mesenchymal stem cells into blood vessel endothelial cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 665-669, 2005.
Article
em Zh
| WPRIM
| ID: wpr-318841
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study differentiation of human bone marrow-derived mesenchymal stem cells (BDMSC) into blood vessel endothelial cells for ideal cell origin of complex organ tissue engineering vascularization and injured tissue repairing by cell transplantation.</p><p><b>METHOD</b>After different days of induction with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in 3D fibrin-gels and matrigel, BDMSC and angiogenesis were determined by the utilization of morphological observation, tissue section and CD34, CD31, vascular endothelial growth factor receptor-1 (VEGFR-1, Flt-1), VEGFR-2 (Flk-1), and vWF that were special for blood vessel endothelial cells.</p><p><b>RESULT</b>After 3D-cultured and induced with VEGF and bFGF in vitro in fibrin-gels and matrigel for 3-21 days, BDMSC expressed CD34, CD31, Flt-1, Flk-1, and vWF came into vessel-like configuration.</p><p><b>CONCLUSION</b>VEGF, bFGF as well as Flt-1 and Flk-1, expressed by BDMSC, may form a feasible microenviroment after induction and play an important role during processes of blood vessel endothelial cell differentiation and vessel-like configuration forming of BDMSC. Mesenchymal stem cells may be applied to tissue engineering vascularization and injured tissue repairing by cell transplantation.</p>
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Fator de von Willebrand
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Fibrina
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Diferenciação Celular
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Fator 2 de Crescimento de Fibroblastos
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Técnicas de Cultura de Células
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Biologia Celular
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Receptor 1 de Fatores de Crescimento do Endotélio Vascular
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Receptor 2 de Fatores de Crescimento do Endotélio Vascular
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Células Endoteliais
Limite:
Humans
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2005
Tipo de documento:
Article