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NS398 induced apoptosis in pancreatic carcinoma cell strain BxPC-3 through a COX-2-in dependent pathway / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 601-605, 2005.
Artigo em Chinês | WPRIM | ID: wpr-318855
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of the selective cyclooxygenase-2 (COX-2) inhibitor NS398 on the growth of human pancreatic tumor BxPC-3 cell strain and its possible mechanisms.</p><p><b>METHODS</b>The effect of NS398 on cell growth was assessed by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl thiazolyl blue (MTT) assay. Apoptosis was determined by fluorescence-activated cell scanning (FACS) analysis and assessment of the floating cell/attached cell ratio. Caspase-3 activation was evaluated by Active Caspase-3 Apoptosis Kit with flow cytometry. Reverse transcriptase-polymerase chain reaction analysis (RT-PCR) and Western blot were used to demonstrate expression levels of COX-1, COX-2 mRNA, and protein, as well as Caspase-3 protein in pancreatic tumor BxPC-3 cell strain.</p><p><b>RESULTS</b>Selective COX-2 inhibitor NS398 significantly decreased cell viability and induced apoptosis in pancreatic tumor BxPC-3 cell strain. The protein expression of Caspase-3 was induced by high-concentration NS398. Caspase-3 activity was strongly activated by NS398.</p><p><b>CONCLUSIONS</b>Selective COX-2 inhibitor NS398 has antiproliferative and proapoptotic potential in pancreatic tumor BxPC-3 cells. Such effect is independent of COX-2, but correlates with Caspase-3 activation.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Neoplasias Pancreáticas / Patologia / Farmacologia / Sulfonamidas / Células Tumorais Cultivadas / Inibidores de Ciclo-Oxigenase / Apoptose / Ciclo-Oxigenase 1 / Ciclo-Oxigenase 2 / Caspase 3 Limite: Humanos Idioma: Chinês Revista: Acta Academiae Medicinae Sinicae Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Neoplasias Pancreáticas / Patologia / Farmacologia / Sulfonamidas / Células Tumorais Cultivadas / Inibidores de Ciclo-Oxigenase / Apoptose / Ciclo-Oxigenase 1 / Ciclo-Oxigenase 2 / Caspase 3 Limite: Humanos Idioma: Chinês Revista: Acta Academiae Medicinae Sinicae Ano de publicação: 2005 Tipo de documento: Artigo