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Negative inotropic effect of meperidine in rat ventricular muscle and the underlying mechanism / 生理学报
Acta Physiologica Sinica ; (6): 197-200, 2003.
Artigo em Chinês | WPRIM | ID: wpr-318917
ABSTRACT
The purpose of the present study was to investigate the effect of meperidine on rat ventricular muscle. Cardiac function was assessed in Langendorff-perfused rat hearts and intracellular calcium level was recorded in enzymatically isolated rat ventricular myocytes using spectrofluorometric techniques. To explore the underlying mechanism, whole-cell configuration of patch-clamp technique was used to record L-type Ca(2+) current. The results showed that meperidine decreased the product of heart rate and left ventricular developed pressure (LVDP HR), maximal rate of the left ventricular pressure increase (LV +dP/dt(max)) and decrease (LV -dP/dt(max)), but increased left ventricular end-diastolic pressure in a dose-dependent manner (0-1000 micromol/L). Meperidine also produced a dose-dependent reduction in electrically induced [Ca(2+)](i) transient amplitude and an increase in diastolic [Ca(2+)](i) baseline level, but did not alter the caffeine (20 mmol/L) induced Ca(2+) release from intracellular ryanodine-sensitive Ca(2+) stores. Meperidine at 100 micromol/L inhibited L-type Ca(2+) current to 67.4 10.1% of control but did not affect the voltage dependency of activation and inactivation. The inhibitory effect of meperidine on Ca(2+) current could not be prevented by pretreatment with the opioid receptor antagonist naloxone. These data suggest that meperidine exerts a negative inotropic effect by inhibiting L-type Ca(2+) current. The lack of effect of naloxone implies that the action is independent of the opioid receptor.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Função Ventricular Esquerda / Ratos Sprague-Dawley / Técnicas de Patch-Clamp / Canais de Cálcio Tipo L / Miócitos Cardíacos / Depressão Química / Relação Dose-Resposta a Droga / Frequência Cardíaca / Meperidina Limite: Animais Idioma: Chinês Revista: Acta Physiologica Sinica Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Função Ventricular Esquerda / Ratos Sprague-Dawley / Técnicas de Patch-Clamp / Canais de Cálcio Tipo L / Miócitos Cardíacos / Depressão Química / Relação Dose-Resposta a Droga / Frequência Cardíaca / Meperidina Limite: Animais Idioma: Chinês Revista: Acta Physiologica Sinica Ano de publicação: 2003 Tipo de documento: Artigo