Effect of protein kinase C on K(V) channel in rat bronchial smooth muscle / 生理学报
Acta Physiologica Sinica
;
(6): 135-141, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-318928
ABSTRACT
The effect of protein kinase C (PKC) signaling pathway on the activity of voltage-dependent delayed rectifier potassium channel (K(V)) and the expression of K(V) isoform K(V)1.5 in rat bronchial smooth cells (BSMCs) were investigated with whole-cell patch clamp, Western-blot and RT-PCR techniques. The results showed (1) phorbol 12-myristate 13-acetate (PMA), a PKC activator, caused a significant inhibition of K(V) channel currents in rat BSMCs. The inhibition was partly abolished by Ro31-8220, a PKC inhibitor. (2) PMA caused a significant suppression of the expression of K(V)1.5 mRNA and protein in rat BSMCs. These effects were attenuated by Ro31-8220. The results suggest that in rat BSMCs PKC activation inhibits K(V) currents and down-regulates the expression of K(V)1.5.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Proteína Quinase C
/
Brônquios
/
RNA Mensageiro
/
Acetato de Tetradecanoilforbol
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Técnicas de Patch-Clamp
/
Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2003
Tipo de documento:
Artigo
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