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Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells / 生理学报
Acta Physiologica Sinica ; (6): 71-74, 2003.
Artigo em Chinês | WPRIM | ID: wpr-318940
ABSTRACT
The newly discovered endogenous vasodilating and diuretic peptide adrenomedullin (AM) was considered to be of attractive value in clinical treatment of hypertension and congestive heart failure. In order to explore the treatment of cardiovascular diseases by expressing AM in vivo, AM cDNA was inserted into mammalian expressing vector pcDNA3.1, and in vitro expression of AM was carried out in cultured K(562) cell line. AM mRNA was amplified by RT-PCR from the total RNA isolated from the adrenal glands of rats and was inserted into pcDNA3.1 vector to form pcDNA3.1AM, the recombinant pcDNA3.1AM was then transferred into cultured K(562) cell line by liposome. The expression of AM in pcDNA3.1AM transferred cell was identified by RT-PCR and dot immunoblot assay. The results demonstrated that there were AM mRNA in the pcDNA3.1AM-transferred K(562) cell line and AM peptides in the culturing medium, indicating that the recombinant pcDNA3.1AM vector can express AM in mammalian cell line.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Recombinantes / RNA Mensageiro / Transfecção / Células K562 / Adrenomedulina / Reação em Cadeia da Polimerase em Tempo Real / Vetores Genéticos / Genética Limite: Animais / Humanos Idioma: Chinês Revista: Acta Physiologica Sinica Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Recombinantes / RNA Mensageiro / Transfecção / Células K562 / Adrenomedulina / Reação em Cadeia da Polimerase em Tempo Real / Vetores Genéticos / Genética Limite: Animais / Humanos Idioma: Chinês Revista: Acta Physiologica Sinica Ano de publicação: 2003 Tipo de documento: Artigo