Effect of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptosis and thermosensitivity of BcaCD885 cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 112-114, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-319044
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptosis and thermosensitivity of BcaCD885 cells.</p><p><b>METHODS</b>After phosphorothioate antisense Bcl-xL oligodeoxynucleotides were transfected into BcaCD885 cells. The characteristics of apoptotic cells were evaluated by morphological observation and TUNEL staining. Apoptotic rate and Bcl-xL protein expression were analyzed with flow cytometry. The influence of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptotic rate of BcaCD885 cells induced by hyperthermia with 43 degrees C 40 min was also examined through flow cytometry.</p><p><b>RESULTS</b>The BcaCD885 cells transfected with phosphorothioate antisense Bcl-xL oligodeoxynucleotides displayed apoptotic morphological features. The Bcl-xL protein expression level of these cells was down-regulated significantly compared with the controlled group (P < 0.05). The apoptotic rate of these cells induced by hyperthermia was increased significantly compared with the controlled group (P < 0.05).</p><p><b>CONCLUSION</b>Phosphorothioate antisense Bcl-xL oligodeoxynucleotides can induce apoptosis and improve thermosensitivity of BcaCD885 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Tionucleotídeos
/
Neoplasias Bucais
/
Células Tumorais Cultivadas
/
Transfecção
/
Regulação Neoplásica da Expressão Gênica
/
Oligonucleotídeos Antissenso
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2004
Tipo de documento:
Artigo
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