Transfection of human endostatin gene with lipofectamin and the expression of hES protein in Tca8113 cell / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 96-99, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-319049
ABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to establish transfergeneic Tca8113 cell and evaluate the expression of human endostatin (hES) gene in the cell colone in vitro.</p><p><b>METHODS</b>To transfect hES gene into Tca8113 cells, lipofectamin was complexed with plasmid encoding hES gene, and blasticidin S antibiotic was adopted to select Tca8113--hES cell clone. Immunohistochemistry S-P method was adopted to detect the expression of hES in the transfergenic Tca8113 cell in vitro.</p><p><b>RESULTS</b>Transfected by hES, the transfergenic Tca8113 cells could grow and proliferate in RPMI--1640 culture medium containing blasticidin S antibiotic. The expression rate of hES reached 100%.</p><p><b>CONCLUSION</b>hES gene can express in hES-transfected Tca8113 cell in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias da Língua
/
Células Tumorais Cultivadas
/
Carcinoma de Células Escamosas
/
Transfecção
/
Divisão Celular
/
Clonagem Molecular
/
Endostatinas
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2004
Tipo de documento:
Artigo
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