Expression of Rho-kinase and heme oxygenase in the corpus cavernosum of spontaneous hypertensive rats / 中华男科学杂志
National Journal of Andrology
;
(12): 215-219, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-319243
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of Rho-kinase and heme oxygenase and the interaction among NOS/NO, HO/CO and RhoA/Rho-kinase in the corpus cavernosum of spontaneous hypertensive rats (SHRs).</p><p><b>METHODS</b>A series of electric stimuli were applied to the corpus cavernosum nerves of 7 SHRs, the changes of ICP/MAP observed continuously and the expressions of ROCK2, HO-2 and eNOS analyzed by Western blot and immunohistochemistry. Another 7 WKY rats were taken as controls.</p><p><b>RESULTS</b>Compared with the controls, ICP/MAP did not rise obviously (P < 0.05), HO-2 dropped sharply (P = 0.006) and the expression of the ROCK2 protein was elevated markedly (P = 0.017) in the SHRs. Immunohistochemistry showed HO-2 to be distributed in the smooth muscle cells and nervous cells of the corpus cavernosum, and eNOS mainly in the vascular endothelial cells. The expressions of HO-2 and eNOS were obviously reduced in the SHRs.</p><p><b>CONCLUSION</b>NOS/NO, HO/CO and RhoA/Rho-kinase are related with erectile dysfunction in SHRs and may interact on one another.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pênis
/
Ratos Endogâmicos SHR
/
Ratos Endogâmicos WKY
/
Imuno-Histoquímica
/
Western Blotting
/
Quinases Associadas a rho
/
Heme Oxigenase (Desciclizante)
/
Hipertensão
/
Disfunção Erétil
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2008
Tipo de documento:
Artigo
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