A morphological study with serial histological slices on the normal and abnormal gubernacula in newborn male mice / 中华男科学杂志
National Journal of Andrology
;
(12): 206-210, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-319245
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of serial slices microscopic histological investigation for the elaborate evaluation of reproductive system malformations.</p><p><b>METHODS</b>Newborn male mice prenatally exposed to different doses of subcutaneously given diethylstilbestrol (DES) from gestational day 9 to 17 were treated by fixing parts of the abdomen in situ and setting them to transected serial slices. All the slices were stained, studied under the microscope and serially recorded by software. The gubernaculum was morphologically analyzed and its location and size were measured.</p><p><b>RESULTS</b>Morphologically, the gubernaculum could be identified clearly, its structure inhomogeneous from proximal to distal and dissymmetric from right to left. The environmental estrogen produced different effects on the morphology of the gubernaculum in different parts and most obviously affected its length.</p><p><b>CONCLUSION</b>Prenatal exposure to environmental estrogen has evident and general effects on the gubernacular development of newborn male mice. The morphological study with serial histological slices gives a precise and systematic evaluation of genital malformations.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Efeitos Tardios da Exposição Pré-Natal
/
Testículo
/
Anormalidades Urogenitais
/
Carcinógenos
/
Idade Gestacional
/
Dietilestilbestrol
/
Toxicidade
/
Animais Recém-Nascidos
/
Camundongos Endogâmicos
Limite:
Animais
/
Gravidez
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS