Construction of the recombinant adenovirus vector bearing dual-survivin shRNA / 中华男科学杂志
National Journal of Andrology
;
(12): 324-327, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-319260
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).</p><p><b>METHODS</b>Dual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.</p><p><b>RESULTS</b>The recombinant adenovirus vector was constructed and the target sequence was obtained.</p><p><b>CONCLUSION</b>The construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Transfecção
/
Enzimas de Restrição do DNA
/
Linhagem Celular
/
Adenoviridae
/
Reação em Cadeia da Polimerase
/
RNA Interferente Pequeno
/
Proteínas de Fluorescência Verde
/
Proteínas Inibidoras de Apoptose
/
Vetores Genéticos
Limite:
Humanos
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2008
Tipo de documento:
Artigo
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