Evaluation of sperm mitochondrial membrane potential by JC-1 fluorescent staining and flow cytometry / 中华男科学杂志
National Journal of Andrology
; (12): 135-138, 2008.
Article
em Zh
| WPRIM
| ID: wpr-319268
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility and clinical significance of detecting sperm mitochondrial membrane potential (MMP) by JC-1 fluorescent staining and flow cytometry, and to explore the relationship between the results of JC-1 staining and seminal parameters.</p><p><b>METHODS</b>Sixty-three semen samples were divided into a fertile (n = 31) and an infertile group (n = 32) and underwent computer-assisted semen analysis (CASA). All the samples were washed, followed by JC-1 staining and evaluation of sperm MMP by flow cytometry. The percentage of normal sperm MMP was indicated as the percentage of sperm emitting orange-red fluorescence (JC-1 + %).</p><p><b>RESULTS</b>The JC-1 + % was significantly higher in the fertile group than in the infertile one ([75.89 +/- 15.69]% vs [54.04 +/- 22.21] %, P = 0.000), correlated positively with sperm motility (r = 0.610, P = 0.000) and the percentage of grade a + b sperm (r = 0.614, P = 0.000) and negatively with grade d sperm (r = -0.504, P = 0.000). There was a significant positive correlation between the results of JC-1 staining (JC-1 + %) and that of Rh123 /PI dual fluorescent staining (Rh123 + / PI (-)%) (r = 0.938, P = 0.000).</p><p><b>CONCLUSION</b>JC-1 staining and flow cytometry could readily and quickly detect sperm MMP and the sperm JC-1 + % could be an auxiliary marker for the diagnosis of male infertility.</p>
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Índice:
WPRIM
Assunto principal:
Fisiologia
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Motilidade dos Espermatozoides
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Espermatozoides
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Coloração e Rotulagem
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Química
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Reprodutibilidade dos Testes
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Potencial da Membrana Mitocondrial
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Citometria de Fluxo
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Corantes Fluorescentes
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Métodos
Limite:
Adult
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Humans
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Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2008
Tipo de documento:
Article