Expression of aquaporin-4 protein in the brain of preeclampsia model rats / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the expression of aquaporin-4 (AQP4) protein in preeclampsia(PE) model rats.</p><p><b>METHODS</b>Adult SD rats were divided into PE model group (n=7) induced by endotoxin, normal pregnancy group (n=6) and non-pregnancy group (n=12) treated with an equal volume of saline. AQP4 protein expression in the brain of the rats was detected with immunohistochemical staining and Western blotting.</p><p><b>RESULTS</b>The blood pressure in PE model rats (135∓9 mmHg) was significantly higher than that in normal pregnancy rats (116∓8 mmHg) and non-pregnancy rats (112∓6 mmHg) (P<0.02). The rats in PE model group showed obvious proteinuria compared with the other two groups (3.8∓0.5 vs 2.6∓0.6 and 2.3∓0.4, P<0.05). Immunohistochemistry showed that AQP4 protein was localized primarily around the brain parenchymal blood vessels. Western analysis revealed a significantly elevated AQP4 protein expression in PE model group (39.6∓4.9) compared with that in normal pregnancy group (26.5∓4.3) and non-pregnancy group (9.7∓2.1) (P<0.01).</p><p><b>CONCLUSION</b>The up-regulation of AQP4 protein around the intraparenchymal blood vessels of the brain may play a role in the development of eclampsia.</p>