Effect of apigenin on proliferation and apoptosis of human lung cancer NCI-H460 cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1137-1140, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-319461
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of apigenin on the proliferation and apoptosis of human lung cancer cell line NCI-H460.</p><p><b>METHODS</b>NCI-H460 cells were cultured with different concentrations of apigenin, and MTT assay was used to evaluate the cell inhibition rates. Apoptosis of NCI-H460 cells was observed under a fluorescence microscope with Hoechst 33258 staining and quantified by flow cytometry using annexin V-FITC/PI stain. The expressions of apoptosis-related proteins Bax, Bcl-2 and caspase-3 were analyzed by Western blotting.</p><p><b>RESULTS</b>Apigenin causes concentration- and time-dependent inhibition of the proliferation of the cells. NCI-H460 cells treated with apigenin showed significant morphological changes of apoptosis, and the cell apoptotic rates increased as apigenin concentration increased. Western blotting demonstrated that apigenin increased the protein levels of Bax and caspase-3 and reduced the protein expression of Bcl-2.</p><p><b>CONCLUSION</b>Apigenin can inhibit the proliferation and induce apoptosis of NCI-H460 cells possibly by up-regulating expression of Bax and caspase-3 and down-regulating the expression of Bcl-2.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Transdução de Sinais
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Linhagem Celular Tumoral
/
Apigenina
/
Proliferação de Células
/
Proteína X Associada a bcl-2
/
Caspase 3
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Artigo
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