β-amyloid protein up-regulates the expression of the receptor for advanced glycation end products by increasing ROS production / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1132-1136, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-319462
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism of β-amyloid protein (Aβ) in regulating the expression of the receptor for advanced glycation end products (RAGE).</p><p><b>METHODS</b>Aβ1-40 was injected into the bilateral hippocampus of rats, and 3 weeks later, the levels of reactive oxygen species (ROS) production were detected by flow cytometry. The expressions of RAGE, reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (gp9l(phox) and p47(phox)), nuclear factor-κB (NF-κB), and inhibitor of κB (IκB) were measured by Western blotting.</p><p><b>RESULTS</b>Injection of Aβ1-40 caused a significant increase in the expressions of RAGE, gp9l(phox), p47(phox), phospho-p47(phox), phospho-IκBα, NF-κB and phospho-NF-κB in rat hippocampus but decreased the level of IκBα. Aβ1-40 injection also resulted in a significantly increased content of ROS in the hippocampus of the rats.</p><p><b>CONCLUSION</b>Aβ up-regulates the expression of RAGE in rat hippocampus via NADPH/ ROS/NF-κB signaling pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fragmentos de Peptídeos
/
Glicoproteínas de Membrana
/
Receptores Imunológicos
/
Transdução de Sinais
/
Regulação para Cima
/
Peptídeos beta-Amiloides
/
NF-kappa B
/
Espécies Reativas de Oxigênio
/
Ratos Sprague-Dawley
/
Estresse Oxidativo
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Artigo
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