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MicroRNA-218 expression and its role in hepatocellular carcinoma / 南方医科大学学报
Journal of Southern Medical University ; (12): 1127-1131, 2013.
Artigo em Chinês | WPRIM | ID: wpr-319463
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of microRNA-218 (miR-218) and its role in hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Forty-six pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-218 expression using qRT-PCR. A miR-218 mimic was transfected into HepG2 cells, and the cell viability and apoptosis were analyzed by MTT assay and flow cytometry, and the potential targets of miR-218 were detected by qRT-PCR and Western blotting.</p><p><b>RESULTS</b>The expressions of miR-218 in HCC tissues were significantly down-regulated compared to those in the adjacent tissues (P<0.05). Down-regulation of miR-218 was found to correlate significantly with the tumor size (>5 cm) and an advanced TNM stage (III+IV) (P<0.05). Ectopic expression of miR-218 in HepG2 cells resulted in suppressed cell proliferation and enhanced cell apoptosis as well as the down-regulation of Bmi-1 and CDK6 mRNA and protein expressions (P<0.05).</p><p><b>CONCLUSION</b>The low-expression of miR-218 is correlated with malignant clinicopathological characteristics of HCC, and miR-218 may inhibit cell proliferation and promote cell apoptosis by down-regulating Bmi-1 and CDK6 in HCC.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Apoptose / Carcinoma Hepatocelular / MicroRNAs / Proliferação de Células / Quinase 6 Dependente de Ciclina / Células Hep G2 / Complexo Repressor Polycomb 1 / Genética / Neoplasias Hepáticas Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Apoptose / Carcinoma Hepatocelular / MicroRNAs / Proliferação de Células / Quinase 6 Dependente de Ciclina / Células Hep G2 / Complexo Repressor Polycomb 1 / Genética / Neoplasias Hepáticas Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2013 Tipo de documento: Artigo