11R-VIVIT inhibits the expression of urokinase-type plasminogen activator receptor in podocytes / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of 11R-VIVIT on lipopolysaccharide (LPS)-induced expression of urokinase-type plasminogen activator receptor (uPAR) in podocytes.</p><p><b>METHODS</b>A LPS-induced proteinuria mouse model and in vitro cultured podocytes treated with LPS were both divided into control group, LPS group and LPS+11 R-VIVIT group. The mRNA and protein expressions of uPAR in mouse kidney tissues and the podocytes were measured by real-time qPCR, laser scanning confocal microscopy and Western blotting.</p><p><b>RESULTS</b>Compared with LPS group, LPS+11 R-VIVIT group showed a significantly lowered urine albumin/creatinine ratio (P<0.001) and markedly reduced mRNA and protein expressions of uPAR (PuPAR mRNA<0.001; PuPAR=0.001).</p><p><b>CONCLUSION</b>11R-VIVIT can ameliorate proteinuria probably by decreasing the expression of uPAR in podocytes.</p>