Phosphorylation of 14-3-3zeta at serine 58 and neurodegeneration following kainic acid-induced excitotoxicity / 대한해부학회지
Anatomy & Cell Biology
;
: 150-156, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-31958
ABSTRACT
Oxidative stress-induced cell death leads to phosphorylation of 14-3-3zeta at serine 58. 14-3-3zeta is detected at significant levels in cerebrospinal fluid after kainic acid (KA)-induced seizures. Here we examined temporal changes in 14-3-3zeta phosphorylation in the hippocampus and amygdala of mice after KA treatment. Mice were killed at 2, 6, 24, or 48 h after KA (30 mg/kg) injection. We observed an increase in TUNEL and Fluoro-Jade B (FJB)-stained neurons in the hippocampus and amygdala of KA-treated mice. Phospho (p)-14-3-3zeta and p-JNK expression was increased in the hippocampus 2 and 6 h after KA treatment, respectively. In immunohistochemical analysis, p-14-3-3zeta-positive cells were present in the CA3 region of the hippocampus and the central nucleus of amygdala (CeA) of KA-treated mice. Thus, phosphorylation of 14-3-3zeta at serine 58 may play an important role in KA-induced hippocampal and amygdaloid neuronal damage.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Convulsões
/
Serina
/
Morte Celular
/
Marcação In Situ das Extremidades Cortadas
/
Fluoresceínas
/
Hipocampo
/
Tonsila do Cerebelo
/
Ácido Caínico
/
Neurônios
Limite:
Animais
Idioma:
Inglês
Revista:
Anatomy & Cell Biology
Ano de publicação:
2010
Tipo de documento:
Artigo
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