Chiral separation of fluvastatin enantiomers with in vitro cellular method / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 12-16, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-319840
ABSTRACT
<p><b>OBJECTIVE</b>To establish a chiral separation method for determination of fluvastatin enantiomer with in vitro cellular model.</p><p><b>METHODS</b>The determination was performed on Chiralpak AD column (4.6 mm × 250 mm); and the phase consisted of hexane-isopropanol-trifluoroacetic acid (90100.1) at a flow rate of 0.5 ml/min with UV detection of 239 nm.</p><p><b>RESULT</b>The standard curve was linear over the concentration range of 20 μmol/L-300 μmol/L (r² = 0.9993, r² = 0.9997). The recovery for this assay was (99.4 ± 0.8)%, precision for inter-assay and intra-assay was <10 %.</p><p><b>CONCLUSION</b>The normal-phase HPLC chiral separation method was accurate and suitable for study on the stereoselectivity of fluvastatin with in vitro cellular model.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Estereoisomerismo
/
Ácidos Graxos Monoinsaturados
/
Células Cultivadas
/
Cromatografia Líquida de Alta Pressão
/
Indóis
/
Métodos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS