Detection of RRM1, ERCC1 and BRCA1 gene expression in non-small cell lung cancer tissues and peripheral blood by SYBR real-time fluorescent quantitative PCR / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 628-633, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-319847
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for the detection of RRM1, ERCC1 and BRCA1 gene expression by SYBR real-time fluorescent quantitative PCR in non-small cell lung cancer tissues and peripheral blood.</p><p><b>METHODS</b>The plasmid standard of RRM1, ERCC1, BRCA1 and β-actin genes was constructed. SYBR real-time PCR was performed, and the standard curve was established. The expressions of RRM1, ERCC1 and BRCA1 mRNA in non-small cell lung cancer tissues and peripheral blood were detected.</p><p><b>RESULT</b>The standard curve presented linearity. The liquate curves of standard gene were all single apex, indicating that a good specificity was obtained.</p><p><b>CONCLUSION</b>The developed SYBR real-time fluorescent quantitative PCR has advantage of convenient operation, low cost, good specificity and high veracity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
RNA Mensageiro
/
Reação em Cadeia da Polimerase
/
Actinas
/
Carcinoma Pulmonar de Células não Pequenas
/
Proteína BRCA1
/
Proteínas Supressoras de Tumor
/
Proteínas de Ligação a DNA
/
Endonucleases
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2010
Tipo de documento:
Artigo
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