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Biological significance of relationship between nuclear localization of Rac1 and progression of gastric carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 676-680, 2011.
Artigo em Chinês | WPRIM | ID: wpr-320108
ABSTRACT
<p><b>OBJECTIVE</b>To observe the subcellular localization of Rac1 and the expression of Tiam1 and Rac1 in gastric carcinoma, in order to reveal the relationship between the distribution of Rac1 and progression of gastric carcinoma.</p><p><b>METHODS</b>Both carcinoma and adjacent normal tissue of 48 patients with gastric carcinoma were studied in this study. Tissue distribution and expression of Rac1 and Tiam1 were analyzed by immunohistochemistry and real-time polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Compared with that of adjacent non-cancerous gastric mucosa, the expression of Rac1 in cancer tissues was significantly increased. The positive rate of Rac1 expression was 18.8% (9/48 cases) in adjacent non-neoplastic gastric and 79.2% (38/48 cases) in cancer tissues. The positive staining was mainly located in the cell nuclei (31 samples). The real-time PCR results demonstrated that the expression levels of Tiam1 and Rac1 mRNA in cancerous tissues with nuclear localization of Rac1 were evidently increased. Furthermore, nuclear localization of Rac1 was associated with tumor stage and metastasis.</p><p><b>CONCLUSIONS</b>The majority of gastric cancer tissues show nuclear dislocalization of Rac1 expression, which may be a sign of abnormal activation of Tiam1-Rac1 pathway. It may suggest enhanced invasion ability of the gastric carcinoma.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Neoplasias Gástricas / RNA Mensageiro / Imuno-Histoquímica / Núcleo Celular / Reação em Cadeia da Polimerase / Progressão da Doença / Proteínas rac1 de Ligação ao GTP / Fatores de Troca do Nucleotídeo Guanina / Proteína 1 Indutora de Invasão e Metástase de Linfoma de Células T Limite: Adulto / Idoso / Aged80 / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Neoplasias Gástricas / RNA Mensageiro / Imuno-Histoquímica / Núcleo Celular / Reação em Cadeia da Polimerase / Progressão da Doença / Proteínas rac1 de Ligação ao GTP / Fatores de Troca do Nucleotídeo Guanina / Proteína 1 Indutora de Invasão e Metástase de Linfoma de Células T Limite: Adulto / Idoso / Aged80 / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2011 Tipo de documento: Artigo