Synergistic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin and N-nitrosodiethylamine on cell malignant transformation / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 323-330, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-320335
ABSTRACT
<p><b>OBJECTIVE</b>The present paper aims to investigate the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and N-nitrosodiethylamine (DEN) on tumorigenesis and its potential mechanism.</p><p><b>METHODS</b>The potentials of TCDD and DEN in separation or in combination to induce malignant transformation were tested in Balb/c 3T3 cells by using a cell transformation assay method. The possible mechanism of observed effects was studied further by adding α-naphthoflavone (α-NF), a competitive binding agent of TCDD, to the Aryl hydrocarbon receptor (AhR) pathway. The mRNA expressions of Cyp1a1 and Cyp2a5 gene in Balb/c 3T3 cells treated by DEN and TCDD in separation or in combination with or without presence of α-NF were measured with fluorescence quantification RT-PCR technique.</p><p><b>RESULTS</b>The cell transformation frequency (TF) was significantly higher in case of induction with TCDD in combination with DEN, as compared to that with either TCDD or DEN alone. These effects were not inhibited via α-NF. The mRNA expression levels of both Cyp1a1 and Cyp2a5 were enhanced by TCDD treatment alone, but this inducible effect was blocked in cells treated by TCDD and DEN in combination.</p><p><b>CONCLUSION</b>TCDD and DEN had a significant synergistic effect on tumorigenesis when they were used in combination. AhR pathway may not be the key mechanism of this synergistic effect. Thus, it is necessary to further test the potential mechanism involved in cancer development.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Carcinógenos
/
Sequência de Bases
/
Transformação Celular Neoplásica
/
Células 3T3
/
Receptores de Hidrocarboneto Arílico
/
Primers do DNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Sistema Enzimático do Citocromo P-450
/
Dietilnitrosamina
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2013
Tipo de documento:
Artigo
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