Comparison of human papillomavirus detection and genotyping with four different prime sets by PCR-sequencing / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 40-47, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-320371
ABSTRACT
<p><b>OBJECTIVE</b>To assess and compare the Human Papillomavirus (HPV) detection efficiency and the potential clinical utility of PCR sequencing-based technology.</p><p><b>METHODS</b>Four HPV consensus primer sets (GP5+/6+, MGP, MY09/11, and PGMY09/11) were used in order to amplify a broad spectrum of HPV types for HPV infection in 325 cervical samples and the PCR products were sequenced afterwards for the HPV genotyping.</p><p><b>RESULTS</b>The HPV-positive rate was 75.4%, of which 35.5% harbored more than one HPV genotype. A total of 36 different genotypes was found, with HPV 16 (24.1%) being the most prevalent, followed by HPV 58 (13.3%) and HPV 52 (9.6%). There were substantial to almost perfect agreements between different primer sets regarding HPV detection efficiency, with the kappa value varying from 0.751 to 0.925, MGP, and PGMY09/11 were the most effective in detecting multiple infections (P < 0.001). With each of the primer sets, a board range of HPV types could be identified, though there were several differences for a few genotypes.</p><p><b>CONCLUSION</b>The substantial agreement between PCR-sequencing and HC2 for the detection of high-risk HPV (kappa=0.761) indicated that PCR-sequencing is also suitable for routine HPV screening.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Reação em Cadeia da Polimerase
/
Infecções por Papillomavirus
/
Alphapapillomavirus
/
Genética
/
Genótipo
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2013
Tipo de documento:
Artigo
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