The reconstruction of bioengineer active corneal stroma and its biocompatibility study / 生物医学工程学杂志

ABSTRACT

This paper aims to explore the biocompatibility of bioengineer active corneal stroma (BACS), as the biological carrier for cornea reconstruction, to provide the basis for future study on clinic application. The cells and immunogenic components of cornea stroma were removed through different extract methods. A complex of functional corneal stroma cells and acellular corneal stroma was used to reconstruct BACS. Their morphological characteristics and ultrastructures were observed with transmission electron microscope. The complex was grafted into interlamellar stromal pockets. Cells were labeled by BrdU to examine the survival and conversion after grafting. The cells could survive and proliferate in acellular corneal stroma. All the nuclei of the corneal stromal cells showed positive labeling with BrdU in the BACS. After 4 weeks, BACS became transparent; after 8 weeks, the bioengineer active cornea stroma was fully reconstructed.