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Construction of an allogenic chimeric mouse model for the study of the behaviors of donor stem cells in vivo / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1444-1450, 2005.
Artigo em Inglês | WPRIM | ID: wpr-320753
ABSTRACT
<p><b>BACKGROUND</b>It is essential to establish an animal model for the elucidation of the biological behaviors of stem cells in vivo. We constructed a chimeric animal model by in utero transplantation for investigation of stem cell transplantation.</p><p><b>METHODS</b>This chimerism was achieved by injecting the stem cells derived from the bone marrow of green fluorescence protein (GFP)-transgenic mice into fetal mice at 13.5 days of gestation. Several methods such as polymerase chain reaction (PCR), real-time PCR, fluorescence-assisted cell sorting (FACS) and fluorescence in situ hybridization (FISH) were used for the observation of donor cells.</p><p><b>RESULTS</b>Under a fluorescence microscope, we observed the GFP cells of donor-origin in a recipient. PCR, FACS analysis and FISH indicated chimerism at various intervals. Real-time PCR indicated that some donor cells existed in chimera for more than 6 months.</p><p><b>CONCLUSIONS</b>Allogenic stem cells may exist in recipients for a long time and this allogenic animal model provides a useful tool for studying the behavior of hematopoietic stem cells and also offers an effective model system for the study of stem cells.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transplante Homólogo / Reação em Cadeia da Polimerase / Hibridização in Situ Fluorescente / Quimeras de Transplante / Transplante de Células-Tronco Hematopoéticas / Modelos Animais / Citometria de Fluxo Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transplante Homólogo / Reação em Cadeia da Polimerase / Hibridização in Situ Fluorescente / Quimeras de Transplante / Transplante de Células-Tronco Hematopoéticas / Modelos Animais / Citometria de Fluxo Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2005 Tipo de documento: Artigo