Expression of imprinted genes related to Beckwith-Wiedemann syndrome in human oocytes and preimplantation embryos / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 265-267, 2005.
Article
em En
| WPRIM
| ID: wpr-321110
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of imprinted genes related to Beckwith-Wiedemann syndrome (BWS) in human oocytes and preimplantation embryos for understanding the relationship between assisted reproductive technology (ART) and BWS.</p><p><b>METHODS</b>Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.</p><p><b>RESULTS</b>Transcripts of P57KIP2 were detected in human oocytes and at all stages of preimplantation embryos. LIT1 was expressed only in stages of 8-cell and blastocyst. Transcripts of TSSC3 could not be detected in human oocytes and preimplantation embryos.</p><p><b>CONCLUSION</b>Transcripts of P57KIP2 and LIT1, imprinted genes related to BWS, were detected in human preimplantation development; ART might affect the epigenetics of imprinted genes in early embryogenesis.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oócitos
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Síndrome de Beckwith-Wiedemann
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Blastocisto
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Proteínas Nucleares
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Impressão Genômica
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Perfilação da Expressão Gênica
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Canais de Potássio de Abertura Dependente da Tensão da Membrana
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Inibidor de Quinase Dependente de Ciclina p57
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Genética
Limite:
Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2005
Tipo de documento:
Article