Study on single nucleotide polymorphism of TLR4 in Chinese population / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 99-101, 2005.
Article
em Zh
| WPRIM
| ID: wpr-321146
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify the single nucleotide polymorphisms(SNPs) in the regulatory and coding regions of human Toll-like receptor 4(TLR4) gene and to search for its new genetic makers.</p><p><b>METHODS</b>The 5' flank region, exons, parts of the introns, as well as 3' flank region of TLR4 gene were sequenced to identify and characterize the SNPs in Chinese population. SNP genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism for 2 highly distributed SNPs.</p><p><b>RESULTS</b>Five novel SNPs were identified through a 4.98 kb sequencing of TLR4 gene. Among them, three were in 5'flank region, two in 3'UTR. In the sample of Han population from Chongqing, the minor allele frequencies of two highly distributed SNPs were 0.266 and 0.404 respectively.</p><p><b>CONCLUSION</b>Sampling analysis in Han population of Chongqing showed that the two highly distributed SNPs of TLR4 were common in Chinese population and could be used for genetic marker of TLR4 gene.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Polimorfismo de Fragmento de Restrição
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Sequência de Bases
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China
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Reação em Cadeia da Polimerase
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Análise de Sequência de DNA
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Polimorfismo de Nucleotídeo Único
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Povo Asiático
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Receptor 4 Toll-Like
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Frequência do Gene
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Genética
Limite:
Adult
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Female
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Humans
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Male
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2005
Tipo de documento:
Article