The multiplex analysis of epigenetic markers and genetic markers by post-digestion mutagenically separated PCR / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 58-60, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-321160
ABSTRACT
<p><b>OBJECTIVE</b>To establish a novel method for the multiplex analysis of the methylation and single nucleotide polymorphism (SNP).</p><p><b>METHODS</b>The imprinted SNP rs220028 was chosen as a model. Genomic DNA, after being digested with methylation sensitive restriction enzyme, were typed by mutagenically separated PCR (MS-PCR). The polymorphism of restriction site was excluded by PCR-RFLP.</p><p><b>RESULTS</b>By post-digestion MS-PCR, the methylated allele was detected selectively, the maternal origin of which was confirmed by pedigree analysis; A=0.5085, G=0.4915,PIC=0.3749.</p><p><b>CONCLUSION</b>The multiplex analysis of methylation markers and SNP can be achieved by post-digestion MS-PCR. The imprinted SNP locus rs220028 is a potentially useful marker in screening Prader-Willi/Angelman syndrome.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Enzimas de Restrição do DNA
/
Marcadores Genéticos
/
Reação em Cadeia da Polimerase
/
Metilação de DNA
/
Polimorfismo de Nucleotídeo Único
/
Genética
/
Metabolismo
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2005
Tipo de documento:
Artigo
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