Expression of heat shock protein 90β and its regulation in the reproductive system of male mice / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the expression and regulation of heat shock protein 90β (HSP90β) in the testis, epididymis and sperms of mice.</p><p><b>METHODS</b>The localization and expression of HSP90β mRNA and protein were investigated in the testis, epididymis and sperms of mice, and the regulation of HSP90β in the male reproductive system was explored.</p><p><b>RESULTS</b>HSP90β was expressed at a higher level in the epididymis than in the testis. In the sperms of the mice, HSP90β was localized in the acrosome area. The expression of HSP90β in mouse epididymis decreased after castration and recovered the normal level after testosterone treatment. HSP90β expression in the testis and epididymis also underwent changes during the postnatal development of the mice.</p><p><b>CONCLUSION</b>HSP90β may play an important role in spermiogenesis and fertilization, and its expression pattern in the epididymis after castration and during the postnatal development suggests its regulation by hormones and development.</p>