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Screening of hepatocyte proteins interacting with hepatitis B virus X protein using CytoTrap yeast two-hybrid technique / 南方医科大学学报
Journal of Southern Medical University ; (12): 486-490, 2013.
Artigo em Chinês | WPRIM | ID: wpr-322017
ABSTRACT
<p><b>OBJECTIVE</b>To screen the hepatocyte proteins that interact with hepatitis B virus X protein (HBx).</p><p><b>METHODS</b>The recombinant plasmid pSos-HBx was constructed by inserting Sos-HBx fragment into the bait vector, and after sequence verification the plasmid was transformed into competent yeast cells. The expression and self-activation of Sos-HBx protein was detected in the yeast cells. The hepatocyte proteins interacting with the bait protein was screened with CytoTrap yeast two-hybrid technique.</p><p><b>RESULTS</b>The reconstructed plasmid harboring HBx gene expressed Sos-HBx protein in the yeast cells without self-activation of the protein. CytoTrap yeast two-hybrid system identified 6 hepatocyte proteins that interacted with HBx, including fibronectin 1, translationally controlled tumor protein, IQ motif and WD repeats 1, follistatin, orosomucoid 1, and disulfide isomerase family A member 3.</p><p><b>CONCLUSION</b>Six HBx-binding hepatocyte proteins have been identified using the CytoTrap yeast two-hybrid system, which provides clues for further investigation of the role of HBx protein in hepatitis and liver cancer.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Proteínas / Transativadores / Técnicas do Sistema de Duplo-Híbrido / Hepatócitos / Domínios e Motivos de Interação entre Proteínas / Vetores Genéticos / Metabolismo Tipo de estudo: Estudo diagnóstico / Estudo prognóstico / Estudo de rastreamento Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Proteínas / Transativadores / Técnicas do Sistema de Duplo-Híbrido / Hepatócitos / Domínios e Motivos de Interação entre Proteínas / Vetores Genéticos / Metabolismo Tipo de estudo: Estudo diagnóstico / Estudo prognóstico / Estudo de rastreamento Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2013 Tipo de documento: Artigo