The expression of nitric oxide synthase in testes of male rat / 中华男科学杂志
National Journal of Andrology
;
(12): 250-252, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-322602
ABSTRACT
<p><b>OBJECTIVES</b>To determine the expression of nitric oxide synthase (NOS) in testis and to investigate the effects of NO on the reproductive function of testis.</p><p><b>METHODS</b>Testes of adult male Sprague-Dawley rats were fixed in 4% paraformaldehyde. The paraffin sections were made as routine. Immunohistochemical ABC method was used to observe the localization of NOS.</p><p><b>RESULTS</b>Endothelia NOS (eNOS), neuronal NOS (nNOS) and inductive NOS (iNOS) were all expressed in Leydig cells. Only eNOS was expressed in peritubular myoid cells, endothelial and smooth muscle cells of blood vessel, while only nNOS expressed in tunica adventitia of testicular blood vessels. The reactive substance distributes in cytoplasm with negative nuclei. Immunoreactivity for eNOS, nNOS and iNOS in all spermatogenic cells was negative.</p><p><b>CONCLUSIONS</b>Three kinds of NOS were all expressed in testis and the distribution of different NOS had a little difference.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Testículo
/
Imuno-Histoquímica
/
Ratos Sprague-Dawley
/
Óxido Nítrico Sintase
/
Óxido Nítrico
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2002
Tipo de documento:
Artigo
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