Effect of recombinant human adenovirus p53 combined with cisplatin on the expression of human lung adenocarcinoma A549 cell gene / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 383-388, 2010.
Article
em Zh
| WPRIM
| ID: wpr-322767
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of recombinant human adenovirus p53 (rAd-p53) combined with cisplatin on the expression of human lung adenocarcinoma A549 cells.</p><p><b>METHODS</b>Human lung adenocarcinoma A549 cells were treated with rAd-p53 combined with cisplatin (combination group) or with cisplatin alone(cisplatin group). The expressions of the cell genes were compared between these two groups and the results were analyzed by SAM software.</p><p><b>RESULT</b>A total of 43 differential genes were found, 15 of which were up-regulated and 28 were down-regulated.</p><p><b>CONCLUSION</b>Following introduction of rAd-p53, many genes regulating cell cycle, proliferation and apoptosis expresses up or down which significantly enhance chemosensitivity and killing efficiency of cisplatin on human lung adenocarcinoma A549 cells.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Transfecção
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Adenocarcinoma
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Adenovírus Humanos
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Genes p53
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Cisplatino
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Perfilação da Expressão Gênica
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Linhagem Celular Tumoral
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2010
Tipo de documento:
Article