Angiogenesis and regulatory factors in rats with BPH induced by testosterone / 中华男科学杂志
National Journal of Andrology
;
(12): 413-418, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-323347
ABSTRACT
<p><b>OBJECTIVE</b>To study angiogenesis and regulatory factors in the proliferated prostatic tissues of Sprague Dawley (SD) rats with BPH induced by testosterone.</p><p><b>METHODS</b>Sixteen castrated SD rats, aged 8 weeks and weighing 200 approximately 250 g, were equally randomized into a model group and a control group, and the BPH model was established by subcutaneous injection of testosterone. Immunohistochemistry and MIAS (micro-image analysis system) were used to test the manifestations of MVD (microvessel density), VEGF (vascular endothelium growth factor), flk-1, endostatin, MMP-2 (matrix metalloproteinase-2) and TIMP-2 (tissue inhibitor of metalloproteinase-2) in the prostatic tissues of both the model and the control groups. Multiple linear regression with the stepwise method was adopted to analyze the data.</p><p><b>RESULTS</b>The manifestations of MVD, VEGF, flk-1, MMP-2, MMP-2/TIMP-2 and VEGF/endostatin in the model group were higher, while that of endostatin was lower than in the control group (P < 0.01), and the manifestation of TIMP-2 showed no statistical difference between the two groups. The regression analysis indicated that MVD was positively correlated to VEGF, VEGF/endostatin and MMP-2/TIMP-2 (r = 0.974, 0.986, 0.982, P < 0.05) and negatively correlated to endostatin (r = - 0.975, P < 0.05) .</p><p><b>CONCLUSION</b>Testosterone could induce BPH in SD rats by increasing MVD and promoting the multiplication of vascular endothelial cells after regradation of basement membrane.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Próstata
/
Hiperplasia Prostática
/
Testosterona
/
Distribuição Aleatória
/
Ratos Sprague-Dawley
/
Inibidor Tecidual de Metaloproteinase-2
/
Metaloproteinase 2 da Matriz
/
Receptor 2 de Fatores de Crescimento do Endotélio Vascular
/
Endostatinas
/
Fator A de Crescimento do Endotélio Vascular
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2005
Tipo de documento:
Artigo
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