Culture and identification of human adult Leydig cells / 中华男科学杂志
National Journal of Andrology
;
(12): 356-358, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-323360
ABSTRACT
<p><b>OBJECTIVE</b>To obtain a simple and effective method to isolate and purify adult Leydig cells.</p><p><b>METHODS</b>The testes of human adults were digested and then the density gradient centrifugation of the cells was performed with four different Percoll concentrations (60%, 34%, 26%, 21%) to isolate Leydig cells, whose characteristics were identified by cytological observation staining, 3beta-HSD staining and detection of hCG and testosterone secretion.</p><p><b>RESULTS</b>High-concentration (> 90%) purified Leydig cells were acquired, and many identification experiments demonstrated the adequate testosterone secretory function of the isolated and purified Leydig cells.</p><p><b>CONCLUSION</b>This method is easy and efficient for the isolation and purification of adult Leydig cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Testosterona
/
Separação Celular
/
Células Cultivadas
/
Povidona
/
Dióxido de Silício
/
Secreções Corporais
/
Biologia Celular
/
Gonadotropina Coriônica
/
Células Intersticiais do Testículo
Tipo de estudo:
Estudo prognóstico
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2005
Tipo de documento:
Artigo
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