Morphological and molecular biological peculiarities of the experimental autoimmune prostatitis rat model / 中华男科学杂志
National Journal of Andrology
;
(12): 290-295, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-323373
ABSTRACT
<p><b>OBJECTIVE</b>To observe the morphological and molecular biological peculiarities of the experimental autoimmune prostatitis (EAP) rat model made by SC purified prostate protein twice with immune adjuvant.</p><p><b>METHODS</b>Male rats were intradermally immunized with a saline extract of male rat prostate glands (RPG) in Freund's complete adjuvant (FCA) and Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. at the 0 and 30th day, and the concentrations of the extract were respectively 5 mg/ml, 10 mg/ml and 15 mg/ml. At the 45th day, the rats were sacrificed and the morphological and molecular biological changes of the prostate specimens were observed to determine the effective concentration of RPG for a successful model.</p><p><b>RESULTS</b>The expression of inflammation genes such as TNF-alpha, IL-1beta, IL-2 and iNOS obviously increased in the high-dosage model group; LM, EM and in situ hybridization revealed appearant chronic inflammation response, but this was not the case in the other two dosage groups.</p><p><b>CONCLUSION</b>15 mg/ml RPG mixed with FCA (11) 1.0 ml SC with Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. was an effective dosage for the successful model in our experiment.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Próstata
/
Prostatite
/
Doenças Autoimunes
/
Proteínas
/
Vacina contra Difteria, Tétano e Coqueluche
/
Adjuvante de Freund
/
Ratos Wistar
/
Modelos Animais de Doenças
/
Alergia e Imunologia
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2005
Tipo de documento:
Artigo
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