Feasibility of muscle-derived cell autotransplantation as a treatment for post-prostatectomy urinary incontinence / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence.</p><p><b>METHODS</b>Skeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining.</p><p><b>RESULTS</b>At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells.</p><p><b>CONCLUSION</b>Autotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence.</p>