Influence of cryoprotectant with glycerol and freezing-thawing procedure on the motility of human sperm / 中华男科学杂志
National Journal of Andrology
;
(12): 204-206, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-323395
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of cryoprotectant with glycerol and the freezing-thawing procedure on the motility of</p><p><b>METHODS</b>The motion characteristics of human sperm from 18 selected specimens were assessed by computer-assisted human sperm. sperm analysis before and after adding hyper-osmolarity cryoprotectant with glycerol and the freeze-thaw procedure, and the data were evaluated in pairs.</p><p><b>RESULTS</b>The adding of cryoprotectant caused an increase in the proportion of rapid linear motile sperms (P < 0.05) and sperm velocities, including VCL, VSL and VAP (P < 0.005). But no changes were observed in the proportion of progressively motile sperms and sperm motility. Compared with the data from pre-frozen samples, velocities of post-thawed sperms and the percentage of motile sperms in each grade significantly declined (P < or = 0.01), so did ALH, while WOB, LIN and STR remained unchanged (P > 0.05). Significant differences in ALH, WOB, LIN and STR were observed only in comparison between the post-thawed and pre-treated samples.</p><p><b>CONCLUSION</b>The number of Grade a sperms and sperm velocity increased after adding hyper-osmolarity cryoprotectant with glycerol. The sperm motile potential was impaired and even entirely destroyed in some cases by cryodamage. The influences on the sperm motion were differently induced in freezing-thawing procedures.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Preservação do Sêmen
/
Motilidade dos Espermatozoides
/
Criopreservação
/
Crioprotetores
/
Congelamento
/
Glicerol
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2005
Tipo de documento:
Artigo
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