Influence of Candida albicans on the motility and ultrastructure of human spermatozoa in vitro / 中华男科学杂志
National Journal of Andrology
;
(12): 179-184, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-323402
ABSTRACT
<p><b>OBJECTIVE</b>To explore the influence of Candida albicans (Ca) on the motility and ultrastructure of human spermatozoa and its possible mechanism.</p><p><b>METHODS</b>Semen samples obtained from 10 healthy volunteers by masturbation were prepared by the swim-up technique and sperm density to 40 x 10(6)/ml. The samples were then inoculated at 37 degrees C with different concentrations of a uropathogenic strain of Ca isolated from an outpatient, with initial fungi/spermatozoa ratios varying among 11 (Group A), 110 (Group B), 1100 (Group C), 11000(Group D), and 110,000 (Group E). And Group F containing Ham's F-10 only was found as the negative control. Motion parameters were analysed by computer-aided sperm analyzer (CASA) at 0 hour, 1 hour, 2 hours and 4 hours respectively. Modalities of spermatozoa and possible adherence and/or agglutination were observed under the light microscope. Finally, all the samples were studied by transmission electron microscopy.</p><p><b>RESULTS</b>Distinct adhesion of spermatozoa to Ca and agglutination were noticed. In all the motion parameters, progressive motility was affected most and dependent upon incubation time and bacterial concentration. Progressive motility showed a significant difference between Group A and the control (P < 0.01). With the prolongation of incubation time, other parameters were showing more and more differences. Analysis by electron microscopy revealed multiple ultrastructural damages.</p><p><b>CONCLUSION</b>Ca significantly inhibits human sperm motility and decreases sperm viability in vitro. Its mechanism is possibly related to Ca's adhesion to human spermatozoa and the impairment inflicted by Ca to sperm ultrastructure.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Motilidade dos Espermatozoides
/
Espermatozoides
/
Técnicas In Vitro
/
Candida albicans
/
Candidíase Vulvovaginal
/
Microbiologia
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2005
Tipo de documento:
Artigo
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