Effects of heme oxygenase-1 on proteins related to apoptosis in INS-1 cells exposed to intermittent high glucose / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2330-2332, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-323669
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of heme oxygenase-1(HO-1) on proteins related to apoptosis in INS-1 cells with exposure to intermittent high glucose.</p><p><b>METHODS</b>INS-1 cells cultured in vitro were divided into control group, persistent high glucose group (PHG), intermittent high glucose group (IHG), CoPP + intermittent high glucose group (CoPP+IHG), and ZnPP+ intermittent high glucose group (ZnPP+IHG). After 72 h of treatment with the corresponding protocols, the cells were examined for expressions of HO-1 protein by Western blotting and for expressions of Bax and Bcl-2 by immunocytochemistry.</p><p><b>RESULTS</b>In comparison with the control group, the cells in both PHG group and IHG group showed significantly increased expressions of HO-1 (P<0.01) and decreased Bcl-2/Bax ratios (P<0.05). The cells in CoPP+ IHG group exhibited a greater HO-1 protein expression but a lower Bcl-2/Bax ratio than those in IHG group (P<0.05) The ZnPP+IHG group demonstrated opposite changes in terms of HO-1, Bax and Bcl-2 expressions compared with the CoPP+IHG group.</p><p><b>CONCLUSION</b>Intermittent high glucose can lower Bcl-2/Bax ratio in INS-1 cells, and HO-1 may protect INS-1 cells against apoptosis possibly by up-regulating the Bcl-2/Bax ratio.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Linhagem Celular
/
Ilhotas Pancreáticas
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Biologia Celular
/
Proteína X Associada a bcl-2
/
Caspase 3
/
Glucose
/
Heme Oxigenase (Desciclizante)
/
Metabolismo
Tipo de estudo:
Guia de Prática Clínica
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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