The effect of protease-activated receptor2 on rat apoptotic cardiomyocytes underwent ischemia reperfusion injury / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 832-836, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-323968
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of protease-activated receptor 2 (PAR-2) on rat apoptotic cardiomyocytes underwent ischemia reperfusion (I/R) injury.</p><p><b>METHODS</b>Healthy male Sprague-Dawley rats were randomly divided into five groups (n = 8 each) sham-operation group, I/R (ligating the left coronary artery for 30 minutes and followed by 120 minutes reperfusion) group and three SLIGRL-NH2 groups treated with intravenous PAR-2 agonist SLIGRL-NH2 at different doses (0.5, 1, 3 mg/kg) 5 minutes before reperfusion. Apoptic cardiomyocytes was detected by TUNEL staining and by DNA ladder on agarose gel electrophoresis. Bax and Bcl-2 expression in myocardium was analyzed by immunohistochemical technique. The mRNA expression of PAR-2 was determined by Real-time quantitative polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>(1) The apoptosis index and the expression of Bcl-2 and Bax were significantly increased in IR group and SLIGRL-NH2 groups than those in sham group (P < 0.05-0.01). (2) Compared with I/R group, the apoptosis index and the expression of Bax were significantly reduced while the expression of Bcl-2 and PAR-2 mRNA were significantly upregulated by SLIGRL-NH2 in a dose-dependent manner. (3) DNA Agarose gel electrophoresis demonstrated that DNA ladder existed in I/R and 0.5 mg/kg SLIGRL-NH2 group, but not in 1, 3 mg/kg SLIGRL-NH2 groups.</p><p><b>CONCLUSIONS</b>PAR-2 agonist SLIGRL-NH2 could reduce myocardial apoptosis by upregulating the Bcl-2 and PAR-2 mRNA level and downregulating Bax expression in a dose-dependent manner in this rat I/R model.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Traumatismo por Reperfusão
/
Ratos Sprague-Dawley
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Miócitos Cardíacos
/
Receptor PAR-2
/
Proteína X Associada a bcl-2
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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