Bone morphogenetic protein-2-induced alpha 2 (I) collagen expression in odontoblastic MDPC-23 cells mediated by Smad proteins / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 386-389, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-324139
ABSTRACT
<p><b>OBJECTIVE</b>To characterize the role of Smads proteins in alpha 2 (I) collagen (COL1A2) gene expression induced by bone morphogenetic protein-2 (BMP-2) in odontoblast cell line MDPC-23.</p><p><b>METHODS</b>Endogenous Smad protein expression was determined by immunocytochemistry. Smads function and their role in COL1A2 gene expression were investigated in cotransfection experiments using promoter-luciferase reporter gene construct.</p><p><b>RESULTS</b>MDPC-23 cells expressed Smad1, Smad5 and Smad6. BMP-2 promoted the activation of COL1A2 promoter reporter construct. Transient overexpression of Smad1 or Smad5 was enhanced, while overexpression of Smad6 inhibited BMP-2-induced COL1A2 promoter activity. BMP-2 inducibility could be blocked by overexpression of Smad1 or Smad5 dominant negative mutant.</p><p><b>CONCLUSIONS</b>Smad signaling is functioning and appears to be involved in BMP-2-induced COL1A2 collagen transcription in MDPC-23. Smad signaling may play an important role in odontoblast differentiation and dentin extracellular matrix formation mediated by BMP-2.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Linhagem Celular
/
Fator de Crescimento Transformador beta
/
Colágeno
/
Proteínas Morfogenéticas Ósseas
/
Biologia Celular
/
Colágeno Tipo I
/
Proteínas Smad
/
Proteína Morfogenética Óssea 2
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2004
Tipo de documento:
Artigo
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