Studying the damages of mouse retina induced by 2,5-hexanedione / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 331-334, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-324269
ABSTRACT
<p><b>OBJECTIVE</b>To study toxic effects of 2,5-hexanedione (2,5-HD) on pathology and lipid peroxidation in mouse retina.</p><p><b>METHODS</b>Forty-eight mice were randomly divided into blank control group (12 mice), negative control group exposed to normal solution (12 mice) and group exposed to 2,5-HD for 2. 4 and 8 weeks, respectively (24 mice) by intraperitoneal injection (2.5% 2,5-HD) at the dose of 400 mg/kg. The pathological changes of mouse retina were examined under light microscope. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in mouse retina were detected.</p><p><b>RESULTS</b>The retinal structure in the blank and negative control groups was normal. In mice exposed to 2,5-HD for 8 weeks, the swelling of outer and inner segments and disorder arrangement of the segments without clear boundary were found. The staining of outer plexiform layers was uneven and the irregular loose structure appeared. The hyperchromatic pyknotic and necrosis nuclei were presented in ganglion cells layer. Compared with the control and blank groups, the activities of SOD gradually and significantly reduced and the concentrations of MDA increased in group exposed to 2,5-HD (P < 0.05).</p><p><b>CONCLUSION</b>2,5-HD can induce the injury of retina tissues of mice, which may be associated with the lipid peroxidation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Retina
/
Superóxido Dismutase
/
Peroxidação de Lipídeos
/
Toxicidade
/
Hexanonas
/
Malondialdeído
/
Metabolismo
/
Camundongos Endogâmicos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2012
Tipo de documento:
Artigo
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