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Effect of Tongxinluo on 7 gene expression profile associated with vascular endothelium injure of rats with deficiency of vital energy or qi stagnation / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2268-2272, 2007.
Artigo em Chinês | WPRIM | ID: wpr-324362
ABSTRACT
<p><b>OBJECTIVE</b>To investigate 7 gene expression profile associated with inflammation and oxidative stress in vascular endothelium injure of rats with deficiency of vital energy or qi stagnation, and the effect of Tongxinluo on gene expression profile.</p><p><b>METHOD</b>The model of vascular endothelium injury of rats with deficiency of vital energy or qi stagnation were established by using high L-methionine, with load-carrying swimming or being fastened, respectively. RT-PCR and SAGE database which is available in NCBI, were used to analyze the changes of 7 gene expression related with inflammation and oxidative stress in endothelium injure and the effect of Tongxinluo on the gene expression profile.</p><p><b>RESULT</b>Compared with control group, the gene expression of inflammation related COX-1, COX-2, oxidative stress related iNOS, SOD and blood vessel vasomotion related eNOS, ECE, increased in deficiency of vital energy group (P < 0.05 or P < 0.01), and the gene expression decreased with Tongxinluo treatment (P < 0.05 or P < 0.01). The gene expression of COX-1, COX-2, iNOS and eNOS, ECE, increased (P < 0.01), but the gene expression of PCS and SOD decreased (P < 0.01), in qi stagnation group, and the disorder of gene expression improved with treatment of Tongxinluo (P < 0.01).</p><p><b>CONCLUSION</b>The 7 gene expression related to vascular endothelium injure were not the same in rat with deficiency of vital energy or qi stagnation, and Tongxinluo could regulate the disorder of the gene expression, protecting vascular endothelium from injure.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Superóxido Dismutase / Ferimentos e Lesões / Medicamentos de Ervas Chinesas / Endotélio Vascular / Metaloendopeptidases / Distribuição Aleatória / Expressão Gênica / Química / Ácido Aspártico Endopeptidases Tipo de estudo: Estudo diagnóstico Limite: Animais Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Superóxido Dismutase / Ferimentos e Lesões / Medicamentos de Ervas Chinesas / Endotélio Vascular / Metaloendopeptidases / Distribuição Aleatória / Expressão Gênica / Química / Ácido Aspártico Endopeptidases Tipo de estudo: Estudo diagnóstico Limite: Animais Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2007 Tipo de documento: Artigo