Induction of type II alveolar epithelial cells apoptosis in mouse by lipopolysaccharide does not require TNF-alpha / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 625-629, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-324377
ABSTRACT
<p><b>OBJECTIVE</b>To examine whether lipopolysaccharide (LPS)-induced apoptosis correlates with TNF-alpha release by type II alveolar epithelial cells (AEC II), whether TNF-alpha knockout (TNF KO) abrogates the induction of apoptosis by LPS and whether TNF-alpha is sufficient to induce apoptosis in this cell type.</p><p><b>METHODS</b>AEC II were isolated from wild type mice and TNF KO mice. Cells were stimulated with LPS or recombinant murine TNF-alpha for 24 h. TNF-alpha in culture supernatant was determined by ELISA following LPS stimulation. Apoptosis was determined by the terminal deoxynucleotidyl transferase end-labeling (TUNEL) assay after treatment with either LPS or TNF-alpha.</p><p><b>RESULTS</b>LPS induced apoptosis in wild type AEC II in a concentration-dependent manner. LPS-induced AEC II apoptosis was accompanied by an 11-fold increase (from 0.073 +/- 0.065 ng/ml in control to 0.94 +/- 0.14 ng/ml in 50 micro g/ml of LPS, P < 0.01) in TNF-alpha release. However, increasing concentrations (5 or 25 ng/ml) of recombinant murine TNF-alpha failed to induce AEC II apoptosis. In addition, apoptosis did occur in AEC II isolated from TNF KO mice following LPS stimulation.</p><p><b>CONCLUSIONS</b>This study confirms that LPS induces TNF-alpha release and apoptosis in murine AEC II in vitro. Exogenous TNF-alpha failed to induce AEC II apoptosis, and apoptosis occurred following LPS stimulation in cells lacking the ability to produce TNF-alpha. Taken together, these results suggest that LPS-induced AEC II apoptosis occurs by a TNF-alpha-independent mechanism.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Alvéolos Pulmonares
/
Células Cultivadas
/
Lipopolissacarídeos
/
Fator de Necrose Tumoral alfa
/
Apoptose
/
Biologia Celular
/
Células Epiteliais
/
Camundongos Endogâmicos C57BL
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2003
Tipo de documento:
Artigo
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