Rapid genetic characterization of a novel Enterobacteria phage and determination of its host recognizing genes / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 884-890, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-324490
ABSTRACT
We isolated a novel Enterobacteria phage IME08 from hospital sewage, then confirmed it was a double-stranded DNA phage by digesting its genetic material with DNase I, RNase A and several restriction endonucleases respectively. BLAST results of random fragments generated by a random PCR cloning method revealed that it belonged to T4-like virus. We subsequently determined the host recognizing genes (g37 and g38) sequence with a PCR-based "genome jumping" protocol based on highly conserved region at 5' terminus of g37 from four other T4-like Bacteriophages (T4, JS98, T2 and K3). These molecular biological methods enabled us to readily characterize the bacteriophage and efficiently determine the sequence of the genes of interest based on very limited conserved sequence information.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
DNA Viral
/
Reação em Cadeia da Polimerase
/
Genoma Viral
/
Clonagem Molecular
/
Bacteriófago T4
/
Escherichia coli
/
Especificidade de Hospedeiro
/
Genética
/
Métodos
Idioma:
Inglês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Artigo
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