Expression and characterization of a new class IIa bacteriocin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 976-982, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-324513
ABSTRACT
NB-Cl gene is a potential class IIa bacteriocin gene. To obtain its soluble expression, NB-C1 was fused with the green fluorescent protein (GFP) gene and a recombinant expression vector plVEX 2.4d-GFP-NB-C1 was constructed, which was transformed into Escherichia coli BL21(DE3) pLysS. The expressed fusion protein GFP-NB-CI was purified by Ni-NTA affinity chromatography and the bioactivity was examined using Listeria monocytogenes as the indicator bacteria. The results showed that the expressed fusion protein GFP-NB-C1 was soluble and the final concentration of the purified fusion protein was 36.1 mg/L E. coli culture and had the purity above 95%. The antimicrobial assay of GFP-NB-C1 was analyzed and showed its high activity against Listeria monocytogenes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Bacteriocinas
/
Proteínas Recombinantes de Fusão
/
Dados de Sequência Molecular
/
Cromatografia de Afinidade
/
Sequência de Aminoácidos
/
Proteínas de Fluorescência Verde
/
Escherichia coli
/
Vetores Genéticos
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Artigo
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