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Development of a killed but metabolically active anthracis vaccine candidate strain / 生物工程学报
Chinese Journal of Biotechnology ; (12): 781-789, 2011.
Artigo em Chinês | WPRIM | ID: wpr-324538
ABSTRACT
Anthrax is a zoonosis caused by Bacillus anthracis, which seriously affects human health. In recent years, a special phenomenon is found that the metabolic active of a bacterium remains after it is killed. To development of a KBMA (killed but metabolically active) Bacillus anthracis vaccine candidate strain, a plasmid pMAD and a recombinase system Cre-loxP were used to knockout the uvrAB gene of B. anthracis AP422 which lacks both of two plasmids pXO1 and pXO2. The results of PCR and RT-PCR shows that uvrAB genes were deleted from B. anthracis AP422 chromosome successfully. The constructed B. anthracis AP422deltauvrAB was inactivated by photochemical treatment (PCT) including an exposure in a long-wave-length ultraviolet (UVA) light and a treatment of 8-Methoxypsoralen (8-MOP), then the metabolic activity were detected by the method of MTS. The results showed that the killed B. anthracis AP422deltauvrAB maintained a highly metabolic activity for at least 4 hours, showing a state of KBMA. The KBMA strain of B. anthracis AP422deltauvrAB provides the prospective vaccine candidate strain for anthrax.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Efeitos da Radiação / Bacillus anthracis / Raios Ultravioleta / Vacinas de Produtos Inativados / Vacinas contra Antraz / Alergia e Imunologia / Técnicas de Inativação de Genes / Genética / Metoxaleno Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Efeitos da Radiação / Bacillus anthracis / Raios Ultravioleta / Vacinas de Produtos Inativados / Vacinas contra Antraz / Alergia e Imunologia / Técnicas de Inativação de Genes / Genética / Metoxaleno Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2011 Tipo de documento: Artigo