Pilot-scale production of recombinant plasmid pUDK-HGF / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 247-252, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-324556
ABSTRACT
pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
DNA Recombinante
/
DNA Super-Helicoidal
/
Projetos Piloto
/
Fator de Crescimento de Hepatócito
/
Escherichia coli
/
Fermentação
/
Vetores Genéticos
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Artigo
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