Determination of zizybeoside II of Ziziphus jujuba by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a quantitative method for determination of zizybeoside II in Ziziphus jujuba.</p><p><b>METHOD</b>The samples were separated at 30 degrees C on a Zorbax SB-C18 column eluted with methanol-water (20 80) as the mobile phase. Flow rate was set at 1.0 mL x min(-1) and the detection wavelength was set at 210 nm.</p><p><b>RESULT</b>The calibration curve was linear within the range from 0.046 to 0.582 microg (r = 0.999 9) and the average recovery was 97.2%. 12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeoside II in Fructus Jujubae were fluctuated from 0.013% to 0.041%.</p><p><b>CONCLUSION</b>The method is simple, repeatable and could be used for the quality control of Z. jujuba.</p>